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KMID : 0364819870250040339
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Korean Journal of Microbiology
1987 Volume.25 No. 4 p.339 ~ p.345
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Isolation and Identification of 8-glucan Degrading Enzyme Producing Bacterium Using coloured 8-glucan
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Abstract
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1
A bacterium K-4-3, producing glucan hydrolyzing eaayme, was isolated from soil and identified to be Bacillus subtilic by its morphological and physiological characteristics. P-glucan was coloured using cibacroa blue 3G-A and cross linked by the addition of 1,4-butaoedioldiglyddyl ether. This substrate was used for the Isolation of 8-glncaaase producing microorganism. The fl-glucan hydrolyzing enzyme activity from Isolated K-43 strain was also measured using the modified substrate. Bacillus subtilis K-4-3 produced the highest extracellalar fl-glucan hydrolyzing activity in the basal medium containing f glucan as a carbon source, peptone and tryptone as a nitrogen source, and magnesium sulfate as an inorganic salt. The optimum temperature and initial pH for f-glucauase production by Bacillus subtilis K-4-3 were 37°C and pH6. The highest enzyme activity was obtained at the culture age of 54 brs with rotary shaking at 37°C. The crude enzyme showed the highest activity at pH 7.5-3.0 and 65 °C.
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